Event Name: Graduate Seminar Series: Cell and Tissue Stream
Graduate Seminar Series for the Institute of Biomaterials and Biomedical Engineering (IBBME). This day is for cell and tissue stream presenters.
Location: Red Seminar Room – Donnelly Building
Presentation Title: Development of Chemically Heterogeneous Polymeric Carriers for Delivery of Peptides
Abstract: This investigation will assess if a lysine-urethane derived polymeric carrier can be designed to locally release chimeric peptides and maintain therapeutic levels for a prolonged effect. Current work in models of atherosclerosis has demonstrated the ability of chimeric peptides to specifically inhibit smooth muscle cell (SMC) migration by antagonizing adhesion receptors, while unaffecting endothelial cell recovery in injured tissues. Atherosclerosis is one of the leading cardiovascular diseases and interventions to date have failed to substantially reduce the rates of re-occlusion. This failure rate is attributed to vascular inflammation and delayed endothelial repair resulting from poor bio-compatibility and the limited effectiveness of current chemotherapeutic agents. A composite of high molecular weight polyethylene-oxide (PEO) and polymeric carriers, which has been shown to have unique anti-inflammatory characteristics and reduced blood clot, paired with the chimeric peptides designed to specifically target SMC migration identified by the Bendeck Lab can provide a better solution. Fabrication techniques (such as spin coating, dip coating and solvent casting) were examined for their ability to deposit multiple thin layers of PEO to establish stable coatings on medical device substrates. An emulsion inversion polymerization will be used to adjust the size of the carrier particles. Initial sizing and bulk properties of the carrier particles were analyzed through dynamic light scattering techniques, while the characterization of the chemical domains will be assessed through x-ray spectroscopy and second ion mass spectrometry. SMC migration assays will be completed to ensure peptide efficacy is unaffected. Spin coated and dip coated high molecular weight PEO on model glass substrates produced multiple micron thin polymeric films. Preliminary results from the emulsion inversion polymerization produced small particle carriers (~200nm diameter) for peptide incorporation. Further experimentation to determine peptide uptake and release from the particles will be conducted to undertake future pharmaco-kinetics studies of the final polymeric system.
Supervisor Name: Dr. Paul Santerre
Year of Study: 2
Program of Study: MASc
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