Miranda Carleton and IBBME UofT

October 10, 2019 @ 12:30 pm – 1:00 pm
Red Seminar Room
Donnelly Building

Event Name: Graduate Seminar Series: Cell and Tissue Stream

Graduate Seminar Series for the Institute of Biomaterials and Biomedical Engineering (IBBME). This day is for cell and tissue stream presenters.

Location: Red Seminar Room – Donnelly Building

Presentation Title: Methacrylic Acod-Based Hydrogel for Endogenous Repair of Skeletal Muscle
Abstract: Skeletal muscle has a remarkable ability to repair itself; however, large muscle injuries cannot heal on their own. The current treatment for large muscle trauma, autologous tissue grafts, is of limited supply and results in scar tissue formation. Regenerative medicine approaches using biomaterials and/or cells are promising, but the resulting muscle function remains well below that of native tissue. Reasons for this impairment include a lack of graft vascularization and/or a persistent “pro-inflammatory” macrophage response. A novel method of enhancing muscle repair is through regenerative biomaterials, such as those containing methacrylic-acid (MAA). In the skin, MAA-based materials bias macrophages towards a “pro-regenerative” phenotype. Moreover, these materials promote new blood vessel formation. As macrophage polarization and vascularization are key skeletal muscle repair processes, it was hypothesized that MAA-based materials will polarize macrophages favourably leading to the regeneration of vascularized, functional muscle. Here, we investigate the ability of a MAA-collagen hydrogel to repair a VML injury. Three weeks after injury, muscles treated with the MAA-collagen hydrogel had larger regenerating muscle fibers compared to muscles treated with no material or collagen. The muscles treated with the MAA-collagen hydrogel also had a higher SMA+ vessel density than the both controls after 3 weeks. This suggests that the MAA-collagen hydrogels are increasing muscle regeneration and vascularization. Further studies are underway to determine the mechanisms behind the regenerative effect of MAA including its ability to polarize macrophages. This material will be a unique treatment for patients who currently do not recover muscle function after injury.
Supervisor Name: Michael Sefton
Year of Study: 4
Program of Study: PhD

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